IL-17+ CD8+ T cell suppression by dimethyl fumarate associates with clinical response in multiple sclerosis
IL-17-producing CD8+ (Tc17) cells are enriched in active lesions of patients with multiple sclerosis (MS), suggesting a role in the pathogenesis of autoimmunity. Here we show that amelioration of MS by dimethyl fumarate (DMF), a mechanistically elusive drug, associates with suppression of Tc17 cells. DMF treatment results in reduced frequency of Tc17, contrary to Th17 cells, and in a decreased ratio of the regulators RORC-to-TBX21, along with a shift towards cytotoxic T lymphocyte gene expression signature in CD8+ T cells from MS patients. Mechanistically, DMF potentiates the PI3K-AKT-FOXO1-T-BET pathway, thereby limiting IL-17 and RORγt expression as well as STAT5-signaling in a glutathione-dependent manner. This results in chromatin remodeling at the Il17 locus. Consequently, T-BET-deficiency in mice or inhibition of PI3K-AKT, STAT5 or reactive oxygen species prevents DMF-mediated Tc17 suppression. Overall, our data disclose a DMF-AKT-T-BET driven immune modulation and suggest putative therapy targets in MS and beyond.
|Persistent URL||dx.doi.org/10.1038/s41467-019-13731-z, hdl.handle.net/1765/122860|
Lückel, C. (Christina), Picard, F. (Felix), Raifer, H. (Hartmann), Campos Carrascosa, L. (Lucia), Guralnik, A. (Anna), Zhang, Y. (Yajuan), … Huber, M. (Magdalena). (2019). IL-17+ CD8+ T cell suppression by dimethyl fumarate associates with clinical response in multiple sclerosis. Nature Communications, 10(1). doi:10.1038/s41467-019-13731-z