Flow cytometric mepacrine fluorescence can be used for the exclusion of platelet dense granule deficiency
Background: δ-storage pool disease (δ-SPD) is a bleeding disorder characterized by a reduced number of platelet-dense granules. The diagnosis of δ-SPD depends on the measurement of platelet ADP content, but this test is time consuming and requires a relatively large blood volume. Flow cytometric analysis of platelet mepacrine uptake is a potential alternative, but this approach lacks validation, which precludes its use in a diagnostic setting. Objectives: To evaluate the performance of platelet mepacrine uptake as a diagnostic test for δ-SPD. Patients/Methods: Mepacrine fluorescence was determined with flow cytometry before and after platelet activation in 156 patients with a suspected platelet function disorder and compared with platelet ADP content as a reference test. Performance was analyzed with a receiver operating characteristic (ROC) curve. Results: Eleven of 156 patients had δ-SPD based on platelet ADP content. Mepacrine fluorescence was inferior to platelet ADP content in identifying patients with δ-SPD, but both mepacrine uptake (area under the ROC curve [AUC] 0.87) and mepacrine release after platelet activation (AUC 0.80) had good discriminative ability. In our tertiary reference center, mepacrine uptake showed high negative predicitive value (97%) with low positive predictive value (35%). Combined with a negative likelihood ratio of 0.1, these data indicate that mepacrine uptake can be used to exclude δ-SPD in patients with a bleeding tendency. Conclusion: Mepacrine fluorescence can be used as a screening tool to exclude δ-SPD in a large number of patients with a suspected platelet function disorder.
|Keywords||blood platelets, flow cytometry, platelet function testing, platelet storage pool deficiency, quinacrine|
|Persistent URL||dx.doi.org/10.1111/jth.14698, hdl.handle.net/1765/123559|
|Journal||Journal of Thrombosis and Haemostasis|
van Asten, I. (Ivar), Blaauwgeers, M. (Maaike), Granneman, L. (Lianne), Heijnen, H.F.G. (Harry F. G.), Kruip, M.J.H.A, Beckers, E.A.M, … Urbanus, R.T. (2019). Flow cytometric mepacrine fluorescence can be used for the exclusion of platelet dense granule deficiency. Journal of Thrombosis and Haemostasis. doi:10.1111/jth.14698