No Evidence for Circulating Retina Specific Autoreactive T-cells in Latent Tuberculosis-associated Uveitis and Sarcoid Uveitis
Purpose: To detect circulating retina-specific autoreactive CD4+ T-cells and antiretinal antibodies (ARA) in latent tuberculosis (TB)-associated uveitis or sarcoid uveitis patients. Methods: The presence of crude retinal extract (RE) autoreactive CD4+ T-cells was determined by a highly sensitive flowcytometric-based technique examining co-expression of CD25 and CD134 (OX40) on RE stimulated PBMC. The presence of ARA in available matched serum samples was assessed by indirect immunofluorescence. Results: No autoreactive CD4+ T-cells against RE could be detected in either latent TB-associated uveitis or sarcoid uveitis patients, while ARA were detected in the serum of the majority (5/6) of latent TB-associated uveitis and all (3/3) sarcoid uveitis patients. Conclusion: Even with the use of this highly sensitive flowcytometric technique circulating retina-specific autoreactive CD4+ T-cells could not be detected. In contrast, ARA were detected in the majority of patients indicating an adaptive humoral immune response toward retinal antigens had occurred.
|Keywords||ANA, ARA, autoantibodies, autoimmunity, CD25, OX40, retina, sarcoidosis, Tuberculosis, uveitis|
|Persistent URL||dx.doi.org/10.1080/09273948.2019.1698752, hdl.handle.net/1765/123923|
|Journal||Ocular Immunology and Inflammation|
Schrijver, B, Hardjosantoso, H. (Hannah), ten Berge, J.C.E.M, Schreurs, M.W.J, Van Hagen, P.M. (P. Martin), Brooimans, R.A, … Dik, W.A. (2020). No Evidence for Circulating Retina Specific Autoreactive T-cells in Latent Tuberculosis-associated Uveitis and Sarcoid Uveitis. Ocular Immunology and Inflammation. doi:10.1080/09273948.2019.1698752