Purpose: To detect circulating retina-specific autoreactive CD4+ T-cells and antiretinal antibodies (ARA) in latent tuberculosis (TB)-associated uveitis or sarcoid uveitis patients. Methods: The presence of crude retinal extract (RE) autoreactive CD4+ T-cells was determined by a highly sensitive flowcytometric-based technique examining co-expression of CD25 and CD134 (OX40) on RE stimulated PBMC. The presence of ARA in available matched serum samples was assessed by indirect immunofluorescence. Results: No autoreactive CD4+ T-cells against RE could be detected in either latent TB-associated uveitis or sarcoid uveitis patients, while ARA were detected in the serum of the majority (5/6) of latent TB-associated uveitis and all (3/3) sarcoid uveitis patients. Conclusion: Even with the use of this highly sensitive flowcytometric technique circulating retina-specific autoreactive CD4+ T-cells could not be detected. In contrast, ARA were detected in the majority of patients indicating an adaptive humoral immune response toward retinal antigens had occurred.

Additional Metadata
Keywords ANA, ARA, autoantibodies, autoimmunity, CD25, OX40, retina, sarcoidosis, Tuberculosis, uveitis
Persistent URL dx.doi.org/10.1080/09273948.2019.1698752, hdl.handle.net/1765/123923
Journal Ocular Immunology and Inflammation
Citation
Schrijver, B, Hardjosantoso, H. (Hannah), ten Berge, J.C.E.M, Schreurs, M.W.J, Van Hagen, P.M. (P. Martin), Brooimans, R.A, … Dik, W.A. (2020). No Evidence for Circulating Retina Specific Autoreactive T-cells in Latent Tuberculosis-associated Uveitis and Sarcoid Uveitis. Ocular Immunology and Inflammation. doi:10.1080/09273948.2019.1698752