Breast cancer cells with distinct E-cadherin status exhibit different 3-D invasion behavior in a novel microfluidic system
Different E-cadherin status in breast cancer is associated with different invasion abilities. However, a relevant in vitro study to study this is still missing from the literature. We developed a microfluidic system to characterize the invasion of three breast cancer cell lines with distinct E-cadherin status in 3D under the influence of a chemotactic gradient. We showed that this system can be used to capture the cells’ migration ability and invasion pattern. Using a perfusion system to control chemotactic gradients and integration of a 3D extracellular matrix, we could mimic two essential components of the tumor microenvironment during cancer invasion.
|Keywords||Breast cancer cell invasion, Chemotactic gradient, E-cadherin, Invasion mode, Microfluidics|
|Conference||22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018|
Eslami Amirabadi, H. (H.), Tuerlings, M. (M.), SahebAli, S. (S.), Luttge, R. (R.), van Donkelaar, C.C, Martens, J.W.M, & Den Toonder, J.M.J. (2018). Breast cancer cells with distinct E-cadherin status exhibit different 3-D invasion behavior in a novel microfluidic system. In 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018 (pp. 1658–1660). Retrieved from http://hdl.handle.net/1765/125140