Different E-cadherin status in breast cancer is associated with different invasion abilities. However, a relevant in vitro study to study this is still missing from the literature. We developed a microfluidic system to characterize the invasion of three breast cancer cell lines with distinct E-cadherin status in 3D under the influence of a chemotactic gradient. We showed that this system can be used to capture the cells’ migration ability and invasion pattern. Using a perfusion system to control chemotactic gradients and integration of a 3D extracellular matrix, we could mimic two essential components of the tumor microenvironment during cancer invasion.

Breast cancer cell invasion, Chemotactic gradient, E-cadherin, Invasion mode, Microfluidics
hdl.handle.net/1765/125140
22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018
Department of Medical Oncology

Eslami Amirabadi, H. (H.), Tuerlings, M. (M.), SahebAli, S. (S.), Luttge, R. (R.), van Donkelaar, C.C, Martens, J.W.M, & Den Toonder, J.M.J. (2018). Breast cancer cells with distinct E-cadherin status exhibit different 3-D invasion behavior in a novel microfluidic system. In 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018 (pp. 1658–1660). Retrieved from http://hdl.handle.net/1765/125140


Additional Files
publisher's information