Comparison of MIC test strip and sensititre YeastOne with the CLSI and EUCAST broth microdilution reference methods for in vitro antifungal susceptibility testing of Cryptococcus neoformans
Antimicrobial Agents and Chemotherapy , Volume 64 - Issue 4
We compared MIC test strip (MTS) and Sensititre YeastOne (SYO) methods with EUCAST and CLSI methods for amphotericin B, 5-fluocytosine, fluconazole, voriconazole, and isavuconazole against 106 Cryptococcus neoformans isolates. The overall essential agreement between the EUCAST and CLSI methods was >72% and >94% at ±1 and ±2 dilutions, respectively. The essential agreements between SYO and EUCAST/CLSI for amphotericin B, 5-flucytosine, fluconazole, and voriconazole were >89/>93% and between MTS and EUCAST/CLSI were >57/>75%. Very major error rates were low for amphotericin B and fluconazole (<3%) and a bit higher for the other drugs (<8%).
|Antifungal susceptibility, CLSI, Cryptococcus neoformans, EUCAST, Liofilchem MIC test strip, Sensititre yeastone|
|Antimicrobial Agents and Chemotherapy|
Delma, F.Z. (Fatima Zohra), Al-Hatmi, A.M.S, Buil, J.B. (Jochem B.), van der Lee, H.A, Tehupeiory-Kooreman, M. (Marlou), Sybren De Hoog, G. (G.), … Verweij, P.E. (2020). Comparison of MIC test strip and sensititre YeastOne with the CLSI and EUCAST broth microdilution reference methods for in vitro antifungal susceptibility testing of Cryptococcus neoformans. Antimicrobial Agents and Chemotherapy, 64(4). doi:10.1128/AAC.02261-19