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W. van Leeuwen (Wibeke), C. Libregts, M. Schalk, J. Veuskens, H.A. Verbrugh (Henri) and A.F. van Belkum (Alex)

2001-01-30

Binary typing of Staphylococcus aureus strains through reversed hybridization using digoxigenin-universal linkage system-labeled bacterial genomic DNA.

Publication

Publication

Journal of Clinical Microbiology , Volume 39 - Issue 1 p. 328- 331

A novel binary typing (BT) procedure, based on reversed hybridization of digoxigenin-universal linkage system-labeled bacterial DNA to strip-immobilized probes, is presented. Chromogenic detection of hybrids was performed. Staphylococcus aureus isolates (n = 20) were analyzed to establish the feasibility of BT. A technically simple and fast procedure has been developed for application in routine microbiology laboratories.

Additional Metadata
Keywords *Bacterial Typing Techniques, *Digoxigenin, Chromogenic Compounds, Comparative Study, DNA Probes/*genetics, DNA, Bacterial/analysis/*genetics, Electrophoresis, Gel, Pulsed-Field, Genome, Bacterial, Humans, Nucleic Acid Hybridization/methods, Random Amplified Polymorphic DNA Technique, Research Support, Non-U.S. Gov't, Staphylococcal Infections/*microbiology, Staphylococcus aureus/*classification/genetics
Persistent URL doi.org/10.1128/JCM.39.1.328-331.2001, hdl.handle.net/1765/12914
Journal Journal of Clinical Microbiology
Organisation Erasmus MC: University Medical Center Rotterdam
Citation
van Leeuwen, W, Libregts, C, Schalk, M, Veuskens, J, Verbrugh, H.A, & van Belkum, A.F. (2001). Binary typing of Staphylococcus aureus strains through reversed hybridization using digoxigenin-universal linkage system-labeled bacterial genomic DNA. Journal of Clinical Microbiology, 39(1), 328–331. doi:10.1128/JCM.39.1.328-331.2001
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