A novel binary typing (BT) procedure, based on reversed hybridization of digoxigenin-universal linkage system-labeled bacterial DNA to strip-immobilized probes, is presented. Chromogenic detection of hybrids was performed. Staphylococcus aureus isolates (n = 20) were analyzed to establish the feasibility of BT. A technically simple and fast procedure has been developed for application in routine microbiology laboratories.

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doi.org/10.1128/JCM.39.1.328-331.2001, hdl.handle.net/1765/12914
Journal of Clinical Microbiology
Erasmus MC: University Medical Center Rotterdam

van Leeuwen, W., Libregts, C., Schalk, M., Veuskens, J., Verbrugh, H., & van Belkum, A. (2001). Binary typing of Staphylococcus aureus strains through reversed hybridization using digoxigenin-universal linkage system-labeled bacterial genomic DNA. Journal of Clinical Microbiology, 39(1), 328–331. doi:10.1128/JCM.39.1.328-331.2001