A novel binary typing (BT) procedure, based on reversed hybridization of digoxigenin-universal linkage system-labeled bacterial DNA to strip-immobilized probes, is presented. Chromogenic detection of hybrids was performed. Staphylococcus aureus isolates (n = 20) were analyzed to establish the feasibility of BT. A technically simple and fast procedure has been developed for application in routine microbiology laboratories.

*Bacterial Typing Techniques, *Digoxigenin, Chromogenic Compounds, Comparative Study, DNA Probes/*genetics, DNA, Bacterial/analysis/*genetics, Electrophoresis, Gel, Pulsed-Field, Genome, Bacterial, Humans, Nucleic Acid Hybridization/methods, Random Amplified Polymorphic DNA Technique, Research Support, Non-U.S. Gov't, Staphylococcal Infections/*microbiology, Staphylococcus aureus/*classification/genetics
dx.doi.org/10.1128/JCM.39.1.328-331.2001, hdl.handle.net/1765/12914
Journal of Clinical Microbiology
Erasmus MC: University Medical Center Rotterdam

van Leeuwen, W, Libregts, C, Schalk, M, Veuskens, J, Verbrugh, H.A, & van Belkum, A.F. (2001). Binary typing of Staphylococcus aureus strains through reversed hybridization using digoxigenin-universal linkage system-labeled bacterial genomic DNA. Journal of Clinical Microbiology, 39(1), 328–331. doi:10.1128/JCM.39.1.328-331.2001