We evaluated the accuracy of the VITEK 2 fully automated system to detect and identify glycopeptide-resistant enterococci (GRE) compared to a reference agar dilution method. The sensitivity of vancomycin susceptibility testing with VITEK 2 for the detection of vanA, vanB, and vanC1 strains was 100%. The sensitivity of vancomycin susceptibility testing of vanC2 strains was 77%. The sensitivity of teicoplanin susceptibility testing of vanA strains was 90%. Of 80 vanC enterococci, 78 (98%) were correctly identified by VITEK 2 as Enterococcus gallinarum/Enterococcus casseliflavus. Since the identification and susceptibility data are produced within 3 and 8 h, respectively, VITEK 2 appears a fast and reliable method for detection of GRE in microbiology laboratories.

Anti-Bacterial Agents/*pharmacology, Bacterial Proteins/genetics, Drug Resistance, Microbial/genetics, Enterococcus/*drug effects/genetics, Gram-Positive Bacterial Infections/*microbiology, Humans, Microbial Sensitivity Tests/*methods, Sensitivity and Specificity, Teicoplanin/*pharmacology, Transferases, Vancomycin Resistance/genetics, Vancomycin/*pharmacology
dx.doi.org/10.1128/JCM.39.1.351-353.2001, hdl.handle.net/1765/12915
Journal of Clinical Microbiology
Erasmus MC: University Medical Center Rotterdam

van den Braak, N.P.W.C.J, Goessens, W.H.F, van Belkum, A.F, Verbrugh, H.A, & Endtz, H.P. (2001). Accuracy of the VITEK 2 system to detect glycopeptide resistance in enterococci. Journal of Clinical Microbiology, 39(1), 351–353. doi:10.1128/JCM.39.1.351-353.2001