We tested a phenyl mannitol broth containing ceftizoxime and aztreonam (PHMB(+)) for detection of methicillin-resistant Staphylococcus aureus (MRSA) with reference MRSA strains and, subsequently, with clinical samples (n = 1,098). All reference MRSA strains induced color change in PHMB(+) after 24 to 72 h of incubation. In a clinical setting, 40 MRSA strains were detected with PHMB(+), compared with only 23 detected with a routine method. Thus, this selective broth significantly (P < 0.001) improved the rate of MRSA detection.

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doi.org/10.1128/JCM.39.7.2660-2662.2001, hdl.handle.net/1765/12929
Journal of Clinical Microbiology
Erasmus MC: University Medical Center Rotterdam

Wertheim, H., Verbrugh, H., van Pelt, C., de Man, P., van Belkum, A., & Vos, M. (2001). Improved detection of methicillin-resistant Staphylococcus aureus using phenyl mannitol broth containing aztreonam and ceftizoxime. Journal of Clinical Microbiology, 39(7), 2660–2662. doi:10.1128/JCM.39.7.2660-2662.2001