In this paper we present a solution for simple implementation of adaptive optics in any existing laser scanning fluorescence microscope. Adaptive optics is implemented by the introduction of a multi-actuator adaptive lens between the microscope body and the objective lens. Correction is performed with a sensorless method by optimizing the quality of the images presented on screen by the microscope software. We present the results acquired on both a commercial linear excitation confocal microscope and a custom made multiphoton excitation microscope.

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Journal Optics Letters
Pozzi, P., Quintavalla, M., Wong, A.B., Borst, J.G.G, Bonora, S., & Verhaegen, M. (2020). Plug-and-play adaptive optics for commercial laser scanning fluorescence microscopes based on an adaptive lens. Optics Letters, 45(13), 3585–3588. doi:10.1364/ol.396998