This thesis proposes sonobactericide, which is the use of ultrasound and exogenous cavitation nuclei, such as microbubbles, either alone or as a therapeutic complement for biofilm eradication or as a theragnostic for bacterial infections. Using infective endocarditis clinical isolates of Staphylococcus aureus, three different translational in vitro biofilm models were developed and used in this thesis. Observed sonobactericide effects that can enhance therapy were biofilm disruption with bacterial dispersal, sonoporation (membrane permeabilization), fibrin strand manipulation (bending, recovery, and breaking), and synergistic responses when a thrombolytic and/or antibiotic were present. This thesis paves the way towards the clinical translation of sonobactericide as an effective treatment, diagnostic, or theranostic strategy employed in infectious disease care.

A.F.W. van der Steen (Ton) , N. de Jong (Nico) , K. Kooiman (Klazina) , W.J.B. van Wamel (Willem)
Erasmus University Rotterdam
978-94-6421-182-5
hdl.handle.net/1765/131894
For copyright reasons there is a partial embargo for this dissertation
Department of Biomedical Engineering

Lattwein, K.R. (2021, April 14). Sonobactericide. Erasmus University Rotterdam. Retrieved from http://hdl.handle.net/1765/131894