Optimization of Pancreatic Juice Collection: A First Step Toward Biomarker Discovery and Early Detection of Pancreatic Cancer
American Journal of Gastroenterology , Volume 115 - Issue 12 p. 2103- 2108
INTRODUCTION: Imaging-based surveillance programs fail to detect pancreatic ductal adenocarcinoma at a curable stage, creating an urgent need for diagnostic biomarkers. METHODS: Secretin-stimulated pancreatic juice (PJ) was collected from the duodenal lumen during endoscopic ultrasound. The yield of biomarkers and organoids was compared for 2 collection techniques (endoscope suction channel vs catheter-based) and 3 periods (0-4 vs 4-8 vs 8-15 minutes). RESULTS: Collection through the endoscope suction channel was superior to collection with a catheter. Collection beyond 8 minutes reduced biomarker yield. PJ-derived organoid culture was feasible. DISCUSSION: The optimal protocol for secretin-stimulated PJ collection is through the endoscope suction channel for 8 minutes allowing biomarker detection and organoid culture.
|American Journal of Gastroenterology|
|Organisation||Department of Gastroenterology & Hepatology|
Levink, I.J.M. (Iris J M), Nesteruk, K. (Kateryna), Visser, D.I. (Dido I.), Sieuwerts, A.M, Fernandes, C.J.C. (Celio J C), Jansen, M.P.H.M, … Bruno, M.J. (2020). Optimization of Pancreatic Juice Collection: A First Step Toward Biomarker Discovery and Early Detection of Pancreatic Cancer. American Journal of Gastroenterology, 115(12), 2103–2108. doi:10.14309/ajg.0000000000000939