The work presented in this thesis aims to contribute to understanding of the molecular mechanisms that underlie UV-damage recognition. If not recognized and repaired, damage can result in DNA mutation, which might result in uncontrolled cell growth (cancer) or cell death. The biological relevance of UV-damage repair is emphasised by the severe clinical features associated with three rare autosomal-recessive inherited syndromes: xeroderma pigmentosum (XP), Cockayne syndrome (CS), and trichothiodystrophy (TTD). More specifically, it analyzes the architecture of the protein-DNA complexes formed during the repair reaction. To this purpose, individual protein-DNA complexes were visualized by scanning force microscopy (SFM) (Chapter 3 and 4). Chapter 1 briefly introduces UV-damages and the relevance of UV-damage repair mechanisms. In this chapter we introduce some background important to understand nucleotide excision repair NER, highlighting the roles of every factor involved. In addition, a brief overview of the proteins that are required for damage detection in photoreactivation and NER in humans and Escherichia coli is given. In the Chapter 2 the application of SFM to study the conformation of protein complexes and their functional assemblies on DNA in DNA repair processes, imaging strategies and its challenges are presented.

J.H.J. Hoeijmakers (Jan)
Erasmus University Rotterdam
Erasmus MC: University Medical Center Rotterdam

Janicijevic, A. (2008, June 5). The Architecture of Molecular Complexes Involved in UV-damage Recognition. Erasmus University Rotterdam. Retrieved from