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L. Ugahary (Luana), W.W.J. van de Sande (Wendy), J.C. van Meurs (Jan) and A.F. van Belkum (Alex)

2004-11-01

An unexpected experimental pitfall in the molecular diagnosis of bacterial endophthalmitis.

Publication

Publication

Journal of Clinical Microbiology , Volume 42 - Issue 11 p. 5403- 5405

General primer-mediated ribosomal DNA amplification during endophthalmitis may improve the quality of diagnostic microbiology. However, extreme care needs to be taken not to introduce contaminating bacterial DNA during surgery procedures. The use of decontaminating iodine solutions can lead to such contamination due to the presence of DNA from Pseudomonas-like organisms.

Additional Metadata
Keywords *Equipment Contamination, Anti-Infective Agents, Local/*chemistry, DNA, Bacterial/*analysis/genetics, Endophthalmitis/*diagnosis/microbiology, Eye Infections, Bacterial/*diagnosis/microbiology, Humans, Povidone-Iodine/*chemistry, Pseudomonas/chemistry/genetics, Vitrectomy
Persistent URL doi.org/10.1128/JCM.42.11.5403-5405.2004, hdl.handle.net/1765/13548
Journal Journal of Clinical Microbiology
Organisation Erasmus MC: University Medical Center Rotterdam
Citation
Ugahary, L., van de Sande, W., van Meurs, J., & van Belkum, A. (2004). An unexpected experimental pitfall in the molecular diagnosis of bacterial endophthalmitis. Journal of Clinical Microbiology, 42(11), 5403–5405. doi:10.1128/JCM.42.11.5403-5405.2004
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