General primer-mediated ribosomal DNA amplification during endophthalmitis may improve the quality of diagnostic microbiology. However, extreme care needs to be taken not to introduce contaminating bacterial DNA during surgery procedures. The use of decontaminating iodine solutions can lead to such contamination due to the presence of DNA from Pseudomonas-like organisms.

*Equipment Contamination, Anti-Infective Agents, Local/*chemistry, DNA, Bacterial/*analysis/genetics, Endophthalmitis/*diagnosis/microbiology, Eye Infections, Bacterial/*diagnosis/microbiology, Humans, Povidone-Iodine/*chemistry, Pseudomonas/chemistry/genetics, Vitrectomy
dx.doi.org/10.1128/JCM.42.11.5403-5405.2004, hdl.handle.net/1765/13548
Journal of Clinical Microbiology
Erasmus MC: University Medical Center Rotterdam

Ugahary, L, van de Sande, W.W.J, van Meurs, J.C, & van Belkum, A.F. (2004). An unexpected experimental pitfall in the molecular diagnosis of bacterial endophthalmitis. Journal of Clinical Microbiology, 42(11), 5403–5405. doi:10.1128/JCM.42.11.5403-5405.2004