<p>Isolated myofibers offer the possibility of in vitro study of satellite cells in their niche. We describe a mouse myofiber isolation assay to assess satellite cell activation by quantifying myofiber-derived satellite cell progeny. The assay allows isolation of myofibers from a mouse using standard equipment and reagents. It can be used to compare satellite cells across different mouse models or to evaluate their response to treatments, offering a valuable complementary tool for in vitro experimentation.</p>

doi.org/10.1016/j.xpro.2021.100482, hdl.handle.net/1765/135807
STAR Protocols
Erasmus MC: University Medical Center Rotterdam

R. (Rodrigo) Canibano Fraile, E.L. (Emma) Boertjes, SD Bozhilova, W.W.M. (Pim) Pijnappel, & G.J. (Gerben) Schaaf. (2021). An in vitro assay to quantify satellite cell activation using isolated mouse myofibers. STAR Protocols, 2(2). doi:10.1016/j.xpro.2021.100482