<p>Systems vaccinology has seldomly been used in therapeutic HIV-1 vaccine research. Our aim was to identify early gene ‘signatures’ that predicted virus load control after analytical therapy interruption (ATI) in participants of a dendritic cell-based HIV-1 vaccine trial (DCV2). mRNA and miRNA were extracted from frozen post-vaccination PBMC samples; gene expression was determined by microarray method. In gene set enrichment analysis, responders showed an up-regulation of 14 gene sets (TNF-alpha/NFkB pathway, inflammatory response, the complement system, Il6 and Il2 JAK-STAT signaling, among others) and a down-regulation of 7 gene sets (such as E2F targets or interferon alpha response). The expression of genes regulated by three (miR-223-3p, miR-1183 and miR-8063) of the 9 differentially expressed miRNAs was significantly down-regulated in responders. The deregulation of certain gene sets related to inflammatory processes seems fundamental for viral control, and certain miRNAs may be important in fine-tuning these processes.</p>

doi.org/10.3390/vaccines9070799, hdl.handle.net/1765/135929
Erasmus MC: University Medical Center Rotterdam

Csaba Fehér, Roque Pastor-Lbáñez, Lorna Leal, Montserrat Plana, Mireia Arnedo, HJ (Henk-Jan) van den Ham, … Felipe García. (2021). Association of transcriptomic signatures of inflammatory response with viral control after dendritic cell-based therapeutic vaccination in hiv-1 infected individuals. Vaccines, 9(7). doi:10.3390/vaccines9070799