<p>Bone marrow (BM) mesenchymal stromal cells play an important role in regulating stem cell quiescence and homeostasis; they are also key contributors to various hematological malignancies. However, human bone marrow stromal cells are difficult to isolate and prone to damage during isolation. This protocol describes a combination of mechanical and enzymatic isolation of BM stromal cells from human BM biopsies, followed by FACS sorting to separate stromal sub-populations including mesenchymal stromal cells, fibroblasts, and Schwann cells for single-cell RNA sequencing. For complete details on the use and execution of this protocol, please refer to Leimkühler et al. (2020).</p>

doi.org/10.1016/j.xpro.2021.100538, hdl.handle.net/1765/136198
STAR Protocols
Erasmus MC: University Medical Center Rotterdam

H.F.E. (Hélène) Gleitz - Broeders, I.A.M. (Inge) Snoeren, S.N.R. (Stijn) Fuchs, NB (Nils) Leimkühler, & R.K.M. (Rebekka) Schneider. (2021). Isolation of human bone marrow stromal cells from bone marrow biopsies for single-cell RNA sequencing. STAR Protocols, 2(2). doi:10.1016/j.xpro.2021.100538