RATIONALE: Tumor hypoxia has both prognostic and therapeutic consequences for solid tumors. We developed a novel noninvasive technique, differential path-length spectroscopy (DPS), which allows the measurement of hypoxia-related parameters in the superficial microvasculature of tissue. OBJECTIVES: The aim of this study was to measure the microvascular oxygenation of histologically normal endobronchial mucosa and of neoplastic lesions during bronchoscopy using DPS. METHODS: Sixty-four patients with known or suspected malignancies of the lung were studied. One hundred and five endobronchial lesions (38 histologically normal, 37 metaplastic/mild dysplastic lesions, and 30 invasive carcinomas) were detected by white and/or autofluorescence bronchoscopy and measured using DPS. RESULTS: We observed that bronchial tumors are characterized by a lower blood oxygen saturation and a higher blood content than normal mucosa. No differences were observed between normal and metaplastic/mild dysplastic mucosa. CONCLUSION: DPS is a new optical technique allowing the noninvasive study of endobronchial tumor hypoxia.

Aged, Anoxia/etiology/*metabolism/pathology, Bronchial Neoplasms/blood supply/complications/*metabolism/pathology, Bronchoscopy/*methods, Female, Humans, Male, Oxygen/*metabolism, Respiratory Mucosa/blood supply/*metabolism/pathology, Spectrometry, Fluorescence/methods
dx.doi.org/10.1164/rccm.200501-046OC, hdl.handle.net/1765/13700
American Journal of Respiratory and Critical Care Medicine
Free full text at PubMed
Erasmus MC: University Medical Center Rotterdam

Bard, M.P.L, Amelink, A, Hegt, V.N, Graveland, W.J, Sterenborg, H.J.C.M, Hoogsteden, H.C, & Aerts, J.G.J.V. (2005). Measurement of hypoxia-related parameters in bronchial mucosa by use of optical spectroscopy. American Journal of Respiratory and Critical Care Medicine, 171(10), 1178–1184. doi:10.1164/rccm.200501-046OC