We recently established that two midgestation-derived stromal clones--UG26-1B6, urogenital ridge-derived, and EL08-1D2, embryonic liver-derived--support the maintenance of murine adult bone marrow and human cord blood hematopoietic repopulating stem cells (HSCs). In this study, we investigate whether direct HSC-stroma contact is required for this stem cell maintenance. Adult bone marrow ckit+ Ly-6C- side population (K6-SP) cells and stromal cells were cocultured under contact or noncontact conditions. These experiments showed that HSCs were maintained for at least 4 weeks in culture and that direct contact between HSCs and stromal cells was not required. To find out which factors might be involved in HSC maintenance, we compared the gene expression profile of EL08-1D2 and UG26-1B6 with four HSC-nonsupportive clones. We found that EL08-1D2 and UG26-1B6 both expressed 21 genes at a higher level, including the putative secreted factors fibroblast growth factor-7, insulin-like growth factor-binding proteins 3 and 4, pleiotrophin, pentaxin-related, and thrombospondin 2, whereas 11 genes, including GPX-3 and HSP27, were expressed at a lower level. In summary, we show for the first time long-term maintenance of adult bone marrow HSCs in stroma noncontact cultures and identify some secreted molecules that may be involved in this support.

Animals, Bone Marrow Cells/cytology, C-Reactive Protein/metabolism, Carrier Proteins/metabolism, Cell Culture Techniques/methods, Cell Line, Cells, Cultured, Coculture Techniques, Cytokines/metabolism, DNA Primers/chemistry, Embryo, Mammalian/*cytology, Enzyme-Linked Immunosorbent Assay, Fetal Blood/cytology, Fibroblast Growth Factor 7, Fibroblast Growth Factors/metabolism, Glutathione Peroxidase/metabolism, Heat-Shock Proteins/metabolism, Hematopoietic Stem Cells/*cytology, Humans, Insulin-Like Growth Factor Binding Protein 3/metabolism, Insulin-Like Growth Factor Binding Protein 4/metabolism, Liver/cytology/embryology, Mice, Mice, Inbred C57BL, Mice, Inbred CBA, Neoplasm Proteins/metabolism, Nerve Tissue Proteins/metabolism, Oligonucleotide Array Sequence Analysis, Protein Binding, RNA, Messenger/metabolism, RNA/metabolism, Reverse Transcriptase Polymerase Chain Reaction, Stem Cells/cytology, Stromal Cells/*cytology, Thrombospondins/metabolism, Time Factors
dx.doi.org/10.1634/stemcells.2004-0120, hdl.handle.net/1765/13810
Stem Cells: the international journal of cell differentiation and proliferation
Erasmus MC: University Medical Center Rotterdam

Oostendorp, R.A, Robin, C, Steinhoff, C, Marz, S, Brauer, R, Nuber, U.A, … Peschel, C. (2005). Long-term maintenance of hematopoietic stem cells does not require contact with embryo-derived stromal cells in cocultures. Stem Cells: the international journal of cell differentiation and proliferation, 23(6), 842–851. doi:10.1634/stemcells.2004-0120