Early changes of the portal tract on microcomputed tomography images in a newly-developed rat model for Budd-Chiari syndrome
Background and Aim: The effect of increased sinusoidal pressure on the portal tract in Budd-Chiari syndrome (BCS) is as yet not elucidated. Our aim was to investigate portal changes in a newly-developed rat model for BCS. Methods: We created an outflow obstruction in Sprague-Dawley rats (n = 6) by diameter reduction of the inferior vena cava. Left and right liver lobes with portal vein contrast were scanned using microcomputed tomography, and volumes of the portal tree and liver parenchyma were computed by the ANALYZE software program. Results: Portal branching density was significantly lower in BCS than the shams, and decreased over time (P < 0.01). There was a significant drop in volume of both parenchyma and the portal tree in the left but not right lobes. At 6 weeks post-surgery, the perfusion index (i.e. ratio between both volumes) became equal to (left) or even higher than (right) the shams, suggesting a new equilibrium with preserved portal perfusion. Histological findings were consistent with those observed in humans. Conclusion: As early as day 2, a significant loss of peripheral portal branches was seen, which progressed over time. Inter-lobar differences in vascular abnormalities suggest compensatory mechanisms. Despite a decrease in both liver and portal vein volume, relative portal perfusion appeared spared.
|Keywords||Budd Chiari syndrome, Budd-Chiari syndrome, Circulation, Microcomputed tomography, Portal vein, Rat model, Sprague Dawley rat, animal experiment, animal model, article, computer assisted tomography, computer program, histology, inferior cava vein, inferior cava vein obstruction, liver vein, lung parenchyma, nonhuman, portal vein, portal vein blood, priority journal, rat, vascular disease|
|Persistent URL||dx.doi.org/10.1111/j.1440-1746.2008.05403.x, hdl.handle.net/1765/14647|
|Journal||Journal of Gastroenterology and Hepatology|
Darwish Murad, S, Dom, V.A.L, Ritman, E.L, de Groen, P.C, Beigley, P.E, Abraham, S.C, … Janssen, H.L.A. (2008). Early changes of the portal tract on microcomputed tomography images in a newly-developed rat model for Budd-Chiari syndrome. Journal of Gastroenterology and Hepatology, 23(10), 1561–1566. doi:10.1111/j.1440-1746.2008.05403.x