Monoclonal antibody 11-fibrau: a useful marker to characterize chondrocyte differentiation stage
Biochemical and Biophysical Research Communications , Volume 280 - Issue 3 p. 806- 812
The aim of this study was to determine the feasibility of discriminating between differentiated and dedifferentiated chondrocytes by using the Mab 11-fibrau. Mab 11-fibrau did not bind to differentiated chondrocytes in cartilage of human knee joint, auricle, or nasal septum. During monolayer culture, when cells dedifferentiate, the number of 11-fibrau positive cells gradually increased and reached up to 100% after 4 passages. When differentiated chondrocytes were cultured in alginate, most (90--95%) of the cells remained 11-fibrau negative, in accordance with previous studies demonstrating that differentiated chondrocytes cultured in alginate keep their phenotype. Dedifferentiated (11-fibrau positive) cells were subjected to different redifferentiation regimes. As a well-known fact, cultures in alginate in medium where FCS was replaced by IGF1 and TGF beta 2 results in increased collagen type II formation, indicative for redifferentiation. However, the cells remained 11-fibrau positive, suggesting they are not (yet) fully redifferentiated. On the other hand, when dedifferentiated cells (after 4 passages in monolayer culture) were seeded in a biomaterial and implanted subcutaneously in a nude mouse, the newly formed cartilage matrix contained collagen type II and the 11-fibrau staining on the cells had disappeared. Our results indicate that 11-fibrau may be a reliable and sensitive marker of chondrocyte phenotype.
|Biochemical and Biophysical Research Communications|
|Organisation||Erasmus MC: University Medical Center Rotterdam|
van Osch, G.J.V.M, van der Veen, S.W, Marijnissen, W.J.C.M, & Verhaar, J.A.N. (2001). Monoclonal antibody 11-fibrau: a useful marker to characterize chondrocyte differentiation stage. Biochemical and Biophysical Research Communications, 280(3), 806–812. doi:10.1006/bbrc.2000.4168