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R.S.R. Choudhurry (Roos), D.C. Melles (Damian), K. Eadie (Kimberley), M.C. Vos (Margreet), H.F.L. Wertheim (Heiman), H.A. Verbrugh (Henri), A.F. van Belkum (Alex) and W.B. van Leeuwen (Willem)

2006-05-01

Direct detection of human Staphylococcus aureus carriage in the nose using the Lightcycler Staphylococcus kit

Publication

Publication

Journal of Microbiological Methods , Volume 65 - Issue 2 p. 354- 356

The Lightcycler Staphylococcus kit is a diagnostic tool for direct real-time detection of Staphylococcus aureus in clinical materials. We show here that detection of S. aureus nasal carriage using this test is hampered by competition of DNA from coagulase-negative staphylococci. However the test is well suited for species identification after culture and the identification of high-load S. aureus carriers.

Additional Metadata
Keywords *Reagent Kits, Diagnostic, Carrier State/*microbiology, Humans, Nasal Cavity/*microbiology, Polymerase Chain Reaction/*methods, Sensitivity and Specificity, Staphylococcal Infections/*diagnosis/microbiology, Staphylococcus aureus/genetics/*isolation & purification
Persistent URL doi.org/10.1016/j.mimet.2005.08.009, hdl.handle.net/1765/17753
Series Staphylococcus aureus: Resources
Journal Journal of Microbiological Methods
Organisation Erasmus MC: University Medical Center Rotterdam
Citation
Choudhurry, R., Melles, D., Eadie, K., Vos, M., Wertheim, H., Verbrugh, H., … van Leeuwen, W. (2006). Direct detection of human Staphylococcus aureus carriage in the nose using the Lightcycler Staphylococcus kit. Journal of Microbiological Methods, 65(2), 354–356. doi:10.1016/j.mimet.2005.08.009
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