The Lightcycler Staphylococcus kit is a diagnostic tool for direct real-time detection of Staphylococcus aureus in clinical materials. We show here that detection of S. aureus nasal carriage using this test is hampered by competition of DNA from coagulase-negative staphylococci. However the test is well suited for species identification after culture and the identification of high-load S. aureus carriers.

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Staphylococcus aureus: Resources
Journal of Microbiological Methods
Erasmus MC: University Medical Center Rotterdam

Choudhurry, R.S.R, Melles, D.C, Eadie, K, Vos, M.C, Wertheim, H.F.L, Verbrugh, H.A, … van Leeuwen, W.B. (2006). Direct detection of human Staphylococcus aureus carriage in the nose using the Lightcycler Staphylococcus kit. Journal of Microbiological Methods, 65(2), 354–356. doi:10.1016/j.mimet.2005.08.009