The Lightcycler Staphylococcus kit is a diagnostic tool for direct real-time detection of Staphylococcus aureus in clinical materials. We show here that detection of S. aureus nasal carriage using this test is hampered by competition of DNA from coagulase-negative staphylococci. However the test is well suited for species identification after culture and the identification of high-load S. aureus carriers.

*Reagent Kits, Diagnostic, Carrier State/*microbiology, Humans, Nasal Cavity/*microbiology, Polymerase Chain Reaction/*methods, Sensitivity and Specificity, Staphylococcal Infections/*diagnosis/microbiology, Staphylococcus aureus/genetics/*isolation & purification,
Staphylococcus aureus: Resources
Journal of Microbiological Methods
Erasmus MC: University Medical Center Rotterdam

Choudhurry, R.S.R, Melles, D.C, Eadie, K, Vos, M.C, Wertheim, H.F.L, Verbrugh, H.A, … van Leeuwen, W.B. (2006). Direct detection of human Staphylococcus aureus carriage in the nose using the Lightcycler Staphylococcus kit. Journal of Microbiological Methods, 65(2), 354–356. doi:10.1016/j.mimet.2005.08.009