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K. Kooiman (Klazina), M. Emmer (Marcia), M. Foppen-Harteveld (Miranda), A. van Wamel (Annemieke) and N. de Jong (Nico)

2010

Increasing the endothelial layer permeability through ultrasound-activated microbubbles

Publication

Publication

I E E E Transactions on Biomedical Engineering , Volume 57 - Issue 1 p. 29- 32

Drug delivery to a diseased tissue will be more efficient if the vascular endothelial permeability is increased. Recent studies have shown that the permeability of single cell membranes is increased by ultrasound in combination with contrast agents. It is not known whether this combination can also increase the permeability of an endothelial layer in the absence of cell damage. To investigate the feasibility of controlled increased endothelial layer permeability, we treated monolayers of human umbilical vein endothelial cells with ultrasound and the contrast agent BR14. Barrier function was assessed by measuring transendothelial electrical resistance (TEER). Ultrasound-activated BR14 significantly decreased TEER by 40.3%±3.7% ( p < 0.01). After treatment, no cell detachment or damage was observed. In conclusion, ultrasound-activated BR14 microbubbles increased the endothelial layer permeability. This feature can be used for future ultrasound-guided drug delivery systems.

Additional Metadata
Keywords Absence of cells, Acoustic waves, After-treatment, BR14, Barrier functions, Capillarity, Cell detachment, Cell membranes, Contrast agent, Cytology, Diseased tissues, Drug delivery, Drug delivery system, Electric resistance, Electrical resistances, Endothelial cells, Endothelial layer, Endothelial permeability, Human umbilical vein endothelial cells, Microbubbles, Monolayers, Self assembly, Single cells, Transendothelial electrical resistance (TEER), Ultrasonic transmission, Ultrasonics, Ultrasound contrast agent, article, cell damage, cell membrane permeability, cell structure, controlled study, echo contrast medium, electric resistance, endothelium cell, human, human cell, lactate dehydrogenase, microbubble, microscopy, statistical analysis, tight junction, ultrasound, unclassified drug
Persistent URL doi.org/10.1109/TBME.2009.2030335, hdl.handle.net/1765/19563
Journal I E E E Transactions on Biomedical Engineering
Organisation Erasmus MC: University Medical Center Rotterdam
Citation
Kooiman, K., Emmer, M., Foppen-Harteveld, M., van Wamel, A., & de Jong, N. (2010). Increasing the endothelial layer permeability through ultrasound-activated microbubbles. I E E E Transactions on Biomedical Engineering, 57(1), 29–32. doi:10.1109/TBME.2009.2030335
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