Functional Redundancy of Langerhans Cells and Langerin+ Dermal Dendritic Cells in Contact Hypersensitivity
The Journal of Investigative Dermatology , Volume 130 - Issue 12 p. 2752- 2759
The relative roles of Langerhans cells (LC), dermal dendritic cells (DC), and, in particular, the recently discovered Langerin+ dermal DC subset in the induction and control of contact hypersensitivity (CHS) responses remain controversial. Using an inducible mouse model, in which LC and other Langerin+ DC can be depleted by injection of diphtheria toxin, we previously reported impaired transport of topically applied antigen to draining lymph nodes and reduced CHS in the absence of all Langerin+ skin DC. In this study, we demonstrate that mice with a selective depletion of LC exhibit attenuated CHS only upon sensitization with a low hapten dose but not with a high hapten dose. In contrast, when painting a higher concentration of hapten onto the skin, which leads to increased antigen dissemination into the dermis, CHS is still diminished in mice lacking all Langerin+ skin DC. Taken together, these data suggest that the magnitude of a CHS reaction depends on the number of skin DC, which have access to the hapten, rather than on the presence or absence of a particular skin DC population. LC and (Langerin+) dermal DC thus seem to have a redundant function in regulating CHS.Journal of Investigative Dermatology advance online publication, 12 August 2010; doi:10.1038/jid.2010.223.
|The Journal of Investigative Dermatology|
|Article in press - dd Oktober 2010|
|Organisation||Erasmus MC: University Medical Center Rotterdam|
Noordegraaf, M, Flacher, V, Stoitzner, P, & Clausen, B.E. (2010). Functional Redundancy of Langerhans Cells and Langerin+ Dermal Dendritic Cells in Contact Hypersensitivity. The Journal of Investigative Dermatology, 130(12), 2752–2759. doi:10.1038/jid.2010.223