Myelin-laden macrophages reside within the CNS, the CSF and in the CNS-draining lymph nodes during MS and EAE, suggesting migration of these macrophages between these compartments and interaction with other cells. Since chemokines and their receptors are pivotal for leukocyte trafficking, we addressed whether myelin ingestion affects chemotaxis of mouse macrophages in vitro. Myelin ingestion enhanced expression of CCR7 and CXCR3 on macrophages and migration towards CCL21 and CXCL10. Furthermore, myelin-laden macrophages released chemoattractants resulting in enhanced migration of myeloid cells in vitro. Our data demonstrate that myelin-laden macrophages have increased motility and suggest trafficking between anatomical compartments in vivo. © 2010 Elsevier B.V.

Cerebrospinal fluid, Cervical lymph nodes, Immune cell trafficking, Macrophages, Multiple sclerosis, animal cell, animal experiment, article, bone marrow cell, cell interaction, central nervous system, cerebrospinal fluid, chemokine receptor CCR7, chemokine receptor CXCR3, chemotaxis, controlled study, gamma interferon inducible protein 10, human, in vitro study, lymph node, macrophage function, macrophage migration, mouse, myelin, nonhuman, priority journal, protein expression, secondary lymphoid tissue chemokine,
Journal of Neuroimmunology
Erasmus MC: University Medical Center Rotterdam

van Zwam, M, Wierenga-Wolf, A.F, Melief, M.J, Schrijver, B, Laman, J.D, & Boven, L.A. (2010). Myelin ingestion by macrophages promotes their motility and capacity to recruit myeloid cells. Journal of Neuroimmunology, 225(1-2), 112–117. doi:10.1016/j.jneuroim.2010.04.021