NanoLC-FTICR MS improves proteome coverage attainable for ~3,000 laser microdissected breast carcinoma cells
Proteomics , Volume 7 - Issue 2 p. 323- 329
Proteomics assays hold great promise for unrevealing molecular events that underlie human disease. Effective analysis of clinical samples is essential, but this task is considerably complicated by tissue heterogeneity. Laser capture microdissection (LCM) can be used to selectively isolate target cells from their native tissue environment. However, the small number of cells that is typically procured by LCM severely limits proteome coverage and biomarker discovery potential achievable by conventional proteomics platforms. Herein, we describe the use of nano liquid chromatography-Fourier transform ion cyclotron resonance mass spectrometry for analyzing protein digests of ~3,000 LCM-derived tumor cells from breast carcinoma tissue, corresponding to ~300 ng of total protein. A total of 2,282 peptides were identified by matching LC-MS data to accurate mass and time tag databases that were previously established for human breast (cancer) cell lines. 1,003 unique proteins were confidently identified with 2 or more peptides. Based on Gene Ontology categorization, identified proteins appear to cover a wide variety of biological functions and cellular compartments. This work demonstrates that a substantial number of proteins can be detected and identified from limited number of cells using the AMT tag approach, and opens doors for high throughput in-depth proteomics analysis of clinical samples.
|*Lasers, *Microdissection, *Nanotechnology, *Spectrometry, Mass, Electrospray Ionization, *Spectroscopy, Fourier Transform Infrared, Breast Neoplasms/*metabolism, Carcinoma/*metabolism, Chromatography, Liquid, Female, Humans, Proteome/*analysis/metabolism, breast cancer, laser capture microdissection, mass spectrometry, nLC-FTICR|
|Organisation||Erasmus MC: University Medical Center Rotterdam|
Umar, A, Luider, T.M, Foekens, J.A, & Paša-Tolić, L. (2007). NanoLC-FTICR MS improves proteome coverage attainable for ~3,000 laser microdissected breast carcinoma cells. Proteomics, 7(2), 323–329. doi:10.1002/pmic.200600293