Discovery of better biomarkers for diagnosis, prognosis, and therapy-response prediction is the most critical task of a scientific quest aimed at developing novel, tailor-made therapies for patients with cancer. Consequently, a proteome wide analysis, in addition to genomic studies, is an absolute requirement for a complete functional understanding of tumor biology. Ultra-sensitive, high-performance Fourier transform ion cyclotron resonance (FTICR) mass spectrometry (MS) currently holds an important role in fulfilling the demands of biomarker discovery. In this review, we describe the applicability of FTICR MS for breast cancer proteomics, particularly for the analysis of complex protein mixtures obtained from a limited number of cells typically available from clinical specimens.

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Keywords Fourier transform ion cyclotron resonance, Matrix-assisted laser desorption/ionization, accurate mass and time tag, biomarker discovery, breast cancer, high throughput, laser capture microdissection, nanoscale liquid chromatography, protein arrays
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Journal Expert Review of Proteomics
Umar, A, Jaremko, M, Burgers, P.C, Luider, T.M, Foekens, J.A, & Paša-Tolić, L. (2008). High-throughput proteomics of breast carcinoma cells: a focus on FTICR MS. Expert Review of Proteomics, 5(3), 445–455. doi:10.1586/14789450.5.3.445