1982
Isolation of human oncogene sequences (v-fes homologue) from a cosmid library.
Publication
Publication
Science , Volume 216 p. 1136- 1138
To define the human homolog (or homologs) of transforming sequences (v-fes gene) common to Gardner (GA) and Snyder Theilen (ST) isolates of feline sarcoma virus (FeSV), a representative library of human lung carcinoma DNA in a cosmid vector system was constructed. Three cosmid clones were isolated containing GA/ST FeSV v-fes homologous cellular sequences, within 32- to 42-kilobase cellular inserts representing 56 kilobases of contiguous human cellular DNA. Sequences both homologous to, and colinear with, GA or ST FeSV v-fes are distributed discontinuously over a region of up to 9.5 kilobases and contain a minimum of three regions of nonhomology representing probable introns. A thymidine kinase selection system was used to show that, upon transfection to RAT-2 cells, the human c-fes sequence lacked detectable transforming activity.
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hdl.handle.net/1765/2358 | |
Science | |
Organisation | Erasmus MC: University Medical Center Rotterdam |
Groffen, J., Heisterkamp, N., Grosveld, F., van de Ven, W., & Stephenson, J. R. (1982). Isolation of human oncogene sequences (v-fes homologue) from a cosmid library. Science, 216, 1136–1138. Retrieved from http://hdl.handle.net/1765/2358 |