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M. Busslinger, S. Wright, F.G. Grosveld (Frank), R.A. Flavell (Richard) and E. de Boer (Ernie)

1983

The sequence GGCmCGG is resistant to MspI cleavage

Publication

Publication

Nucleic Acids Research , Volume 11 - Issue 11 p. 3559- 3569

MspI essentially fails to cut the sequence GGCmCGG at enzyme concentrations which give total digestion of CCGG, CmCGG and GGCCGG sites. This result explains why certain sites in mammalian DNA are resistant to both MspI and HpaII and shows that this results from an idiosynchracy of MspI rather than a novel form of DNA methylation at this site in mammalian cells.

Additional Metadata
Keywords 9004-22-2 (Globins), 9007-49-2 (dna), Animals, Base Sequence, Cloning, Molecular, DNA Restriction Enzymes/metabolism, Deoxyribonuclease HpaII, Dna, EC 2.7.1.21 (Thymidine Kinase), EC 3.1.21 (DNA Restriction Enzymes), EC 3.1.21.- (Deoxyribonuclease HpaII), Genes, Structural, Globins/genetics, Human, L Cells (Cell Line)/enzymology, Methylation, Mice, Nucleic Acid Hybridization, Substrate Specificity, Support, Non-U.S. Gov't, Thymidine Kinase/deficiency
Persistent URL hdl.handle.net/1765/2366
Journal Nucleic Acids Research
Organisation Erasmus MC: University Medical Center Rotterdam
Citation
Busslinger, M., Wright, S., Grosveld, F., Flavell, R., & de Boer, E. (1983). The sequence GGCmCGG is resistant to MspI cleavage. Nucleic Acids Research, 11(11), 3559–3569. Retrieved from http://hdl.handle.net/1765/2366
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