We have cloned and determined the nucleotide sequence of the human gene for the neurofilament subunit NF-L. The cloned DNA contains the entire transcriptional unit and generates two mRNAs of approx. 2.6 and 4.3 kb after transfection into mouse L-cells. The NF-L gene has an unexpected intron-exon organization in that it entirely lacks introns at positions found in other members of the intermediate filament gene family. It contains only three introns that do not define protein domains. We discuss possible evolutionary schemes that could explain these results.

*Genes, Structural, 0 (Cosmids), 0 (Genetic Vectors), 0 (Intermediate Filament Proteins), 0 (Macromolecular Systems), 9007-49-2 (dna), Amino Acid Sequence, Base Sequence, Cloning, Molecular, Cosmids, DNA Restriction Enzymes, DNA/analysis, EC 3.1.21 (DNA Restriction Enzymes), Evolution, Exons, Genetic Vectors, Human, Intermediate Filament Proteins/*genetics, Introns, Macromolecular Systems, Support, Non-U.S. Gov't
Biochimica et Biophysica Acta - General Subjects
Erasmus MC: University Medical Center Rotterdam

Julien, J-P, Grosveld, F.G, Yazdanbakhsh, K, Flavell, D, Meijer, D.N, & Mushynski, W.E. (1987). The structure of a human neurofilament gene (NF-L): A unique exon-intron organization in the intermediate filament gene family. Biochimica et Biophysica Acta - General Subjects, 909, 10–20. Retrieved from http://hdl.handle.net/1765/2408