The Locus Control Region (LCR) of the human beta globin gene domain is defined by four erythroid-specific DNasel hypersensitive sites (HSS) located upstream of this multigene cluster. The LCR confers copy number dependent high levels of erythroid specific expression to a linked transgene, independent of the site of integration. To assess the role of the individual hypersensitive sites of the LCR, we have localized HSS4 to a 280bp fragment that is functional both in murine erythroleukaemia (MEL) cells and in transgenic mice. This fragment coincides with the major area of hypersensitivity 'in vivo' and contains a number of DNasel footprints. Bandshift analysis shows that these footprints correspond to binding sites for the erythroid specific proteins GATA1 and NF-E2 and a number of ubiquitous proteins, including jun/fos, Sp1 and TEF2.

*Regulatory Sequences, Nucleic Acid, 9004-22-2 (Globins), 9007-49-2 (dna), Animals, Base Sequence, Binding Sites, Blotting, Southern, Chromosome Mapping, DNA Fingerprinting, DNA/analysis, Deoxyribonuclease I/metabolism, EC 3.1.21.1 (Deoxyribonuclease I), Electrophoresis, Agar Gel, Globins/*genetics, Hela Cells, Human, Mice, Mice, Transgenic, Molecular Sequence Data, Support, Non-U.S. Gov't
hdl.handle.net/1765/2464
Nucleic Acids Research
Erasmus MC: University Medical Center Rotterdam

Pruzina, S, Hanscombe, O, Whyatt, D, Grosveld, F.G, & Philipsen, J.N.J. (1991). Hypersensitive site 4 of the human β-globin locus control region. Nucleic Acids Research, 19, 1413–1419. Retrieved from http://hdl.handle.net/1765/2464