1991
Hypersensitive site 4 of the human β-globin locus control region.
Publication
Publication
Nucleic Acids Research , Volume 19 p. 1413- 1419
The Locus Control Region (LCR) of the human beta globin gene domain is defined by four erythroid-specific DNasel hypersensitive sites (HSS) located upstream of this multigene cluster. The LCR confers copy number dependent high levels of erythroid specific expression to a linked transgene, independent of the site of integration. To assess the role of the individual hypersensitive sites of the LCR, we have localized HSS4 to a 280bp fragment that is functional both in murine erythroleukaemia (MEL) cells and in transgenic mice. This fragment coincides with the major area of hypersensitivity 'in vivo' and contains a number of DNasel footprints. Bandshift analysis shows that these footprints correspond to binding sites for the erythroid specific proteins GATA1 and NF-E2 and a number of ubiquitous proteins, including jun/fos, Sp1 and TEF2.
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hdl.handle.net/1765/2464 | |
Nucleic Acids Research | |
Organisation | Erasmus MC: University Medical Center Rotterdam |
Pruzina, S., Hanscombe, O., Whyatt, D., Grosveld, F., & Philipsen, S. (1991). Hypersensitive site 4 of the human β-globin locus control region. Nucleic Acids Research, 19, 1413–1419. Retrieved from http://hdl.handle.net/1765/2464 |