There is much evidence to suggest that potentially immunogenic tumour cells can escape cytolytic immune destruction by loss of class I antigen expression. Many tumours are allele-specific class I negative and, in murine systems, reconstitution of class I expression by gene transfection leads to an increase in tumour immunogenicity. In many systems where mice have rejected class I transfected tumour cells they are also immune to a subsequent challenge with the untransfected parent tumour. In this study we have examined the effect of stable class II antigen expression (induced by gene transfection) on a class I loss mutant (H-2Kk negative) murine cell line, K36.16. We show that H-2Ek expression is more effective at increasing tumour immunogenicity than the reconstitution of H-2Kk expression in these cells. This suggests that the induction of class II antigen expression on tumour cells may provide an effective way of enhancing tumour-specific immune responses in vivo.

0 (H-2 Antigens), 0 (H-2E(k) antigen, human), 0 (H-2K(K) antigen), Animals, Cell Line, Cytotoxicity, Immunologic/genetics, Genes, MHC Class II/*immunology, H-2 Antigens/*analysis, Immunity/genetics, Leukemia, Experimental/*genetics/immunology, Mice, Mice, Inbred AKR, Support, Non-U.S. Gov't, Transfection/*immunology
hdl.handle.net/1765/2465
Immunology: the journal of cells, molecules, system and technologies
Erasmus MC: University Medical Center Rotterdam

James, R.F.L, Edwards, S, Hui, K.M, Bassett, P.D, & Grosveld, F.G. (1991). The effect of class II gene transfection on the tumourigenicity of the H-2K-negative mouse leukaemia cell line K36.16. Immunology: the journal of cells, molecules, system and technologies, 72, 213–218. Retrieved from http://hdl.handle.net/1765/2465