The minimal requirements for activity in transgenic mice of hypersensitive site 3 of the β globin locus control region.
EMBO Journal , Volume 12 p. 1077- 1085
Proper expression of the human beta-like globin genes is completely dependent on the presence of the locus control region or LCR, a region containing four DNase hypersensitive sites (HS1-4) situated 5' to the structural genes. Linkage of the LCR to a transgene results in copy number-dependent transcription, independent of the site of integration in the host genome. We have analysed a small region of the LCR (HS3) in transgenic animals to determine the minimal interactions that are required for this property. The results show that a specific combination of a G-rich sequence flanked on each side by one binding site for the transcription factor GATA1 is essential to obtain position-independent expression of a linked beta globin gene in erythroid cells. The overall transcriptional activity of HS3 is achieved through synergy with other combinations of similar binding sites.
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|Organisation||Erasmus MC: University Medical Center Rotterdam|
Philipsen, J.N.J, Pruzina, S, & Grosveld, F.G. (1993). The minimal requirements for activity in transgenic mice of hypersensitive site 3 of the β globin locus control region. EMBO Journal, 12, 1077–1085. Retrieved from http://hdl.handle.net/1765/2480