We have examined the chicken Very Low Density Apolipoprotein II (apoVLDL II) gene locus in transgenic mice. A DNA fragment composed of the transcribed region, 16 kb of 5' flanking and 400 bp of 3' flanking sequences contained all the information sufficient for estrogen-inducible, liver-specific expression of the apoVLDL II gene. The far-upstream region contains a Negative Regulating Element coinciding with a DNaseI-hypersensitive site at -11 kb. In transgenic mice, the NRE at -11 kb is used for downregulating the expression to a lower maximum level. The NRE might be used for modulating apoVLDL II gene expression, and may be involved in the rapid shut-down of the expression after hormone removal.

0 (Apolipoproteins), 0 (Estrogens), 0 (Lipoproteins, VLDL), 0 (apolipoprotein VLDL II), Animals, Apolipoproteins/*genetics, Base Sequence, Chickens, Deoxyribonuclease I, EC (Deoxyribonuclease I), Estrogens/physiology, Gene Expression Regulation, Lipoproteins, VLDL/*genetics, Liver/metabolism, Mice, Mice, Inbred C57BL, Mice, Inbred CBA, Mice, Transgenic, Molecular Sequence Data, Organ Specificity, Regulatory Sequences, Nucleic Acid, Support, Non-U.S. Gov't, Support, U.S. Gov't, P.H.S.
Nucleic Acids Research
Erasmus MC: University Medical Center Rotterdam

Wijnholds, J, Philipsen, J.N.J, Pruzina, S, Fraser, P.J, Grosveld, F.G, & Ab, G. (1993). Estrogen-inducible and liver-specific expression of the chicken very low density apolipoprotein II gene locus in transgenic mice. Nucleic Acids Research, 21, 1629–1635. Retrieved from http://hdl.handle.net/1765/2490