Human chorionic gonadotrophin (hCG) is a hormone produced during pregnancy and present at the implantation site and in the maternal blood. Pregnancy has been proposed to represent a controlled state of inflammation at an early stage at the implantation site and later, systemically extended to the maternal circulation. Earlier, we reported that hCG can inhibit the development of diabetes in NOD mice and LPS-induced septic shock in a murine model. We hypothesize that hCG can contribute to the reduction of inflammation by modifying Mφ function. Here, the TG-induced peritonitis model for inflammation was used to investigate the effect of hCG on cytokine production and cell recruitment in vivo. hCG pretreatment in TG-induced peritonitis increased the number of peritoneal cells, especially PMN and monocytes, compared with mice injected with TG only. This increased cell number was partially explained by increased cell survival induced by hCG. Despite the cellular infiltrate, hCG pretreatment decreased i.p. TNF-α, IL-6, PTX3, CCL3, and CCL5 levels. By depleting peritoneal resident Mφ using clodronate liposomes prior to the application of hCG and the TG trigger, we established that Mφ are the main responsive cells to hCG, as the suppressed TNF-α and IL-6 production and increased PMN influx are abolished in their absence. Together, these data suggest that hCG contributes to the controlled inflammatory state of pregnancy by regulating Mφ proinflammatory function.

Cell recruitment, Clodronate liposomes, Resident Mφ, hCG
dx.doi.org/10.1189/jlb.0208126, hdl.handle.net/1765/25356
Journal of Leukocyte Biology
Erasmus MC: University Medical Center Rotterdam

Wan, H, Coppens, J.M.C, van Helden-Meeuwsen, C.G, Leenen, P.J.M, van Rooijen, N, Khan, N.A, … Versnel, M.A. (2009). Chorionic gonadotropin alleviates thioglycollate-induced peritonitis by affecting macrophage function. Journal of Leukocyte Biology, 86(2), 361–370. doi:10.1189/jlb.0208126