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C.C. Hoogenraad (Casper), E. Goedknegt (Erika), E. Hertzberg, A. Neubauer (Andrea), F.G. Grosveld (Frank), N.J. Galjart (Niels) and C.I. de Zeeuw (Chris)

1997-12-01

CLIP-115, a novel brain-specific cytoplasmic linker protein, mediates the localization of dendritic lamellar bodies.

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Neuron , Volume 19 - Issue 6 p. 1187- 1199

Intracellular localization of organelles may depend in part on specific cytoplasmic linker proteins (CLIPs) that link membranous organelles to microtubules. Here, we characterize rat cDNAs encoding a novel, brain-specific CLIP of 115 kDa. This protein contains two N-terminal microtubule-binding domains and a long coiled-coil region; it binds to microtubules and is homologous to CLIP-170, a protein mediating the binding of endosomes to microtubules. CLIP-115 is enriched in the dendritic lamellar body (DLB), a recently discovered organelle predominantly present in bulbous dendritic appendages of neurons linked by dendrodendritic gap junctions. Local microtubule depolymerization leads to a temporary reduction of DLBs. These results suggest that CLIP-115 operates in the control of brain-specific organelle translocations.

Additional Metadata
Keywords 0 (Microtubule-Associated Proteins), 0 (Nerve Tissue Proteins), 0 (Recombinant Proteins), 0 (cytoplasmic linker protein 115), 148349-95-5 (cytoplasmic linker protein 170), 31430-18-9 (Nocodazole), 33069-62-4 (Paclitaxel), Amino Acid Sequence, Animals, Base Sequence, Brain/cytology/*metabolism, COS Cells, Cloning, Molecular, Dendrites/*metabolism/ultrastructure, Human, Male, Microtubule-Associated Proteins/analysis/*biosynthesis/chemistry, Microtubules/drug effects/metabolism/ultrastructure, Molecular Sequence Data, Nerve Tissue Proteins/analysis/*biosynthesis/chemistry, Neurons/cytology/drug effects/*metabolism, Nocodazole/pharmacology, Olivary Nucleus/metabolism, Paclitaxel/pharmacology, Rats, Rats, Wistar, Recombinant Proteins/analysis/biosynthesis, Sequence Alignment, Sequence Homology, Amino Acid, Support, Non-U.S. Gov't, Transfection
Persistent URL doi.org/10.1016/S0896-6273(00)80411-0, hdl.handle.net/1765/2550
Journal Neuron
Organisation Erasmus MC: University Medical Center Rotterdam
Citation
Hoogenraad, C., Goedknegt, E., Hertzberg, E., Neubauer, A., Grosveld, F., Galjart, N., & de Zeeuw, C. (1997). CLIP-115, a novel brain-specific cytoplasmic linker protein, mediates the localization of dendritic lamellar bodies. Neuron, 19(6), 1187–1199. doi:10.1016/S0896-6273(00)80411-0
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