Abstract The GATA-3 transcription factor shows a specific and restricted expression pattern in the developing and adult mouse brain. In the present study we investigated the role of GATA-3 in the caudal raphe system, which is known to operate as a modulator of motor activity. We demonstrate that virtually all neurons in the caudal raphe nuclei that express GATA-3 also produce serotonin. Absence of GATA-3, as analyzed in chimeric -/- mice, affects the cytoarchitecture of serotonergic neurons in the caudal raphe nuclei. As a result the chimeras show a serious defect in their locomotor performance on a rotating rod. In sum, we conclude that GATA-3 plays a major role in the development of the serotonergic neurons of the caudal raphe nuclei, and that it is crucial for their role in locomotion.

Animals, Brain Stem/anatomy & histology/growth & development/metabolism, Chimera, DNA-Binding Proteins/deficiency/genetics/metabolism/*physiology, GATA3 Transcription Factor, Immunohistochemistry, Lac Operon, Mice, Mice, Mutant Strains, Motor Activity/genetics/physiology, Neurons/*metabolism, Raphe Nuclei/cytology/growth & development/*metabolism, Serotonin/*metabolism, Trans-Activators/deficiency/genetics/metabolism/*physiology
The Journal of Neuroscience
Free full text, open at RePub because there is no DOI
Erasmus MC: University Medical Center Rotterdam

van der Wees, J, Karis, A, Goedknegt, E, Rutteman, M, Grosveld, F.G, van Doorninck, J.H, & de Zeeuw, C.I. (1999). GATA-3 is involved in the development of serotonergic neurons in the caudal raphe nuclei. The Journal of Neuroscience, 19(12), 1–8. Retrieved from http://hdl.handle.net/1765/2576