Human MTG16a (CBFA2T3), a chromatin repressor with nucleolar localization, was described to act as a suppressor of breast tumourigenesis. Here we show that MTG16a is a novel ribosomal gene repressor, which can counteract MYC-driven activation of ribosomal RNA (rRNA) transcription. We also show that either knocking down MTG16a by RNA interference, or sequestering MTG16a outside the nucleolus of human breast epithelial cells, hampers acinar morphogenesis concomitant with up-regulation of rRNA synthesis and increased ribogenesis. This is the first demonstration that loss of MTG16a function in the nucleolus of breast epithelial cells can induce morphological and molecular changes typical of breast cancer initiation. © 2009 The Authors Journal compilation

Acinar morphogenesis, Breast cancer, MTG16a (CBFA2T3), Nucleolus, RDNA
dx.doi.org/10.1111/j.1582-4934.2009.00982.x, hdl.handle.net/1765/28599
Journal of Cellular and Molecular Medicine: a journal of translational medicine
Erasmus MC: University Medical Center Rotterdam

Rossetti, S, Hoogeveen, A.T, Esposito, J, & Sacchi, N. (2010). Loss of MTG16a (CBFA2T3), a novel rDNA repressor, leads to increased ribogenesis and disruption of breast acinar morphogenesis. Journal of Cellular and Molecular Medicine: a journal of translational medicine, 14(6 A), 1358–1370. doi:10.1111/j.1582-4934.2009.00982.x