For the formation of lymph nodes and Peyer's patches, lymphoid tissue inducer (LTi) cells are crucial in triggering stromal cells to recruit and retain hematopoietic cells. Although LTi cells have been observed in fetal spleen, not much is known about fetal spleen development and the role of LTi cells in this process. Here, we show that LTi cells collect in a periarteriolar manner in fetal spleen at the periphery of the white pulp anlagen. Expression of the homeostatic chemokines can be detected in stromal and endothelial cells, suggesting that LTi cells are attracted by these chemokines. As lymphotoxin (LT)α1β2can be detected on B cells but not LTi cells in neonatal spleen, starting at 4 days after birth, the earliest formation of the white pulp in fetal spleen occurs in a LTα1β2-independent manner. The postnatal development of the splenic white pulp, involving the influx of T cells, depends on LTα1β2expressed by B cells.

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doi.org/10.1189/jlb.0907659, hdl.handle.net/1765/29758
Journal of Leukocyte Biology
Erasmus MC: University Medical Center Rotterdam

Vondenhoff, M.F.R, Desanti, G.E, Cupedo, T, Bertrand, J.Y, Cumano, A, Kraal, G, … Golub, R. (2008). Separation of splenic red and white pulp occurs before birth in a LTαβ-independent manner. Journal of Leukocyte Biology, 84(1), 152–161. doi:10.1189/jlb.0907659