In this report the genomic characterization of the human excision repair gene ERCC-1 is presented. The gene consists of 10 exons spread over approximately 15 kb. By means of a transfection assay the ERCC-1 promoter was confined to a region of + 170 bp upstream of the transcriptional start site. Classical promoter elements like CAAT, TATA and GC-boxes are absent from this region. Furthermore, ERCC-1 transcription is not UV-inducible. A possible explanation is provided for the previously reported alternative splicing of exon VIII. Analysis of ERCC-1 cDNA clones revealed the occurrence of differential polyadenylation which gives ERCC-1 transcripts of 3.4 and 3.8 kb in addition to the major 1.1 kb mRNA. Apparent evolutionary conservation of differential polyadenylation of ERCC-1 transcripts suggests a possible role for this mode of RNA processing in the ERCC-1 repair function.

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Nucleic Acids Research
Erasmus MC: University Medical Center Rotterdam

van Duin, M., Koken, M., van den Tol, J., ten Dijke, P., Westerveld, A., Bootsma, D., & Hoeijmakers, J. (1987). Genomic characterization of the human DNA excision repair gene ERCC-1. Nucleic Acids Research, 15, 9195–9213. Retrieved from