Flow cytometry (FC) is recognized as an important tool in the diagnosis of myelodysplastic syndromes (MDS) especially when standard criteria fail. A working group within the Dutch Society of Cytometry aimed to implement FC in the diagnostic work-up of MDS. Hereto, guidelines for data acquisition, analysis and interpretation were formulated. Based on discussions on analyses of list mode data files and fresh MDS bone marrow samples and recent literature, the guidelines were modified. Over the years (2005-2011), the concordance between the participating centers increased indicating that the proposed guidelines contributed to a more objective, standardized FC analysis, thereby ratifying the implementation of FC in MDS.

Diagnosis, Flow cytometry, Implementation, MDS, Multicenter analysis, Pitfalls, Standardization
dx.doi.org/10.1016/j.leukres.2011.09.015, hdl.handle.net/1765/30714
Leukemia Research: clinical and laboratory studies
Erasmus MC: University Medical Center Rotterdam

Westers, T.M, van der Velden, V.H.J, Alhan, C, Bekkema, R, Bijkerk, A, Brooimans, R.A, … van de Loosdrecht, A.A. (2012). Implementation of flow cytometry in the diagnostic work-up of myelodysplastic syndromes in a multicenter approach: Report from the Dutch Working Party on Flow Cytometry in MDS. Leukemia Research: clinical and laboratory studies, 36(4), 422–430. doi:10.1016/j.leukres.2011.09.015