We present a method for controlled connection of gold electrodes with dsDNA molecules (locally on a chip) by utilizing polymerase to elongate single-stranded DNA primers attached to the electrodes. Thiol-modified oligonucleotides are directed and immobilized to nanoscale electrodes by means of dielectrophoretic trapping, and extended in a procedure mimicking PCR, finally forming a complete dsDNA molecule bridging the gap between the electrodes. The technique opens up opportunities for building from the bottom-up, for detection and sensing applications, and also for molecular electronics.

doi.org/10.1039/c1nr10518c, hdl.handle.net/1765/31018
Erasmus MC: University Medical Center Rotterdam

Linko, V., Leppiniemi, J., Shen, B., Niskanen, E., Hytönen, V. P., & Toppari, J. J. (2011). Growth of immobilized DNA by polymerase: Bridging nanoelectrodes with individual dsDNA molecules. Nanoscale, 3(9), 3788–3792. doi:10.1039/c1nr10518c