Growth of immobilized DNA by polymerase: Bridging nanoelectrodes with individual dsDNA molecules
Nanoscale , Volume 3 - Issue 9 p. 3788- 3792
We present a method for controlled connection of gold electrodes with dsDNA molecules (locally on a chip) by utilizing polymerase to elongate single-stranded DNA primers attached to the electrodes. Thiol-modified oligonucleotides are directed and immobilized to nanoscale electrodes by means of dielectrophoretic trapping, and extended in a procedure mimicking PCR, finally forming a complete dsDNA molecule bridging the gap between the electrodes. The technique opens up opportunities for building from the bottom-up, for detection and sensing applications, and also for molecular electronics.
|Organisation||Erasmus MC: University Medical Center Rotterdam|
Linko, V, Leppiniemi, J, Shen, B, Niskanen, E, Hytönen, V.P, & Toppari, J.J. (2011). Growth of immobilized DNA by polymerase: Bridging nanoelectrodes with individual dsDNA molecules. Nanoscale, 3(9), 3788–3792. doi:10.1039/c1nr10518c