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E. Soler (Eric), C. Andrieu-Soler (Charlotte), E. de Boer (Ernie), J.C. Bryne, S. Thongjuea (Supat), E.J. Rijkers, J.A.A. Demmers (Jeroen), W.F.J. van IJcken (Wilfred) and F.G. Grosveld (Frank)

2011-02-01

A systems approach to analyze transcription factors in mammalian cells

Publication

Publication

Methods , Volume 53 - Issue 2 p. 151- 162

Transcription factors (TFs) play a central role in the development of multicellular organisms. The sequential actions of critical TFs direct cells to adopt defined differentiation pathways leading to functional, fully differentiated tissues. Here, we describe a generic experimental pipeline that integrates biochemistry, genetics and next generation sequencing with bioinformatics to characterize TF complexes composition, function and target genes at a genome-wide scale. We show an application of this experimental pipeline which aims to unravel the molecular events taking place during hematopoietic cell differentiation.

Additional Metadata
Keywords ChIP-Seq, Erythroid cells, Hematopoiesis, High throughput, Mass spectrometry, Network, Next generation sequencing, Transcription factor
Persistent URL doi.org/10.1016/j.ymeth.2010.08.002, hdl.handle.net/1765/31698
Journal Methods
Organisation Erasmus MC: University Medical Center Rotterdam
Citation
Soler, E., Andrieu-Soler, C., de Boer, E., Bryne, J. C., Thongjuea, S., Rijkers, E. J., … Grosveld, F. (2011). A systems approach to analyze transcription factors in mammalian cells. Methods (Vol. 53, pp. 151–162). doi:10.1016/j.ymeth.2010.08.002


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