university website

A.R. ter Avest (Anja), A.D.M.E. Osterhaus (Albert) and G. van Steenis (Bert)

1987

A comparison of an enzyme-linked immunosorbent assay and counter current electrophoresis for the detection of bovine serum albumin in virus vaccines.

Publication

Publication

Journal of Biological Standardization , Volume 15 - Issue 3 p. 245- 250

A monoclonal antibody directed against bovine serum albumin (BSA) has been developed and used in an enzyme-linked immunosorbent assay (ELISA) system for the detection of BSA in virus vaccines. The results correlated well with those obtained with a counter current electrophoresis system which has been employed routinely for this purpose. The ELISA was slightly more sensitive and more readily applicable to the screening of large numbers of samples but could not be used in the presence of certain stabilizers.

Additional Metadata
Keywords *Counterimmunoelectrophoresis, *Enzyme-Linked Immunosorbent Assay, *Immunoelectrophoresis, 0 (Antibodies, Monoclonal), 0 (Serum Albumin, Bovine), 0 (Viral Vaccines), Animals, Antibodies, Monoclonal/immunology, Cattle, Comparative Study, Drug Contamination/*prevention & control, Hybridomas, Serum Albumin, Bovine/*analysis, Viral Vaccines/analysis/*standards
Persistent URL doi.org/10.1016/0092-1157(87)90027-8, hdl.handle.net/1765/3319
Journal Journal of Biological Standardization
Organisation Erasmus MC: University Medical Center Rotterdam
Citation
ter Avest, A., Osterhaus, A., & van Steenis, B. (1987). A comparison of an enzyme-linked immunosorbent assay and counter current electrophoresis for the detection of bovine serum albumin in virus vaccines. Journal of Biological Standardization, 15(3), 245–250. doi:10.1016/0092-1157(87)90027-8
Free Full Text ( Final Version , 366kb ) cover


User Publication Person Organisation Collection
Close