Comparison of an enzyme-linked immunosorbent assay, an immunofluorescence assay and a hemagglutination inhibition assay for detection of antibodies to K-papovavirus in mice.
Laboratory Animal Science , Volume 39 - Issue 1 p. 21- 24
The sensitivity of a newly developed enzyme-linked immunosorbent assay (ELISA) for detection of antibody to K virus was compared with the sensitivities of an immunofluorescence assay (IFA) and a hemagglutination inhibition assay (HIA). Specific pathogen-free BALB/c RIVM mice, 5 weeks old, were inoculated intraperitoneally with a mouse organ suspension containing 10(4.5) TCID50 of K virus per dose. Control animals were inoculated with a control mouse organ suspension. No clinical signs were observed during the 7 weeks they were followed for the development of serum antibody. The ELISA proved to be the most sensitive of the three assays and demonstrated K virus-specific antibodies as early as 3 days after infection.
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|Laboratory Animal Science|
|Organisation||Erasmus MC: University Medical Center Rotterdam|
Groen, J, Osterhaus, A.D.M.E, Broeders, H.W.J, & Spijkers, H.E.M. (1989). Comparison of an enzyme-linked immunosorbent assay, an immunofluorescence assay and a hemagglutination inhibition assay for detection of antibodies to K-papovavirus in mice. Laboratory Animal Science, 39(1), 21–24. Retrieved from http://hdl.handle.net/1765/3356