Monoclonal anti-idiotypic (anti-Id) antibodies (Ab2) were generated against idiotypes (Id) of canine parvovirus (CPV) specific monoclonal antibodies (MoAbs). The binding of most of these anti-Id antibodies to their corresponding Id could be inhibited by antigen, thus classifying these anti-Id antibodies as Ab2 gamma or Ab2 beta. By inhibiting experiments it was shown that these anti-Id antibodies did not recognize interspecies cross-reactive idiotopes, but recognized private idiotopes, uniquely associated with the Id of the anti-CPV MoAb used for immunization. This classifies these anti-Id antibodies as non-internal image Ab2 gamma. The potential use of these non-internal image anti-Id antibodies for the induction of antiviral antibodies in the CPV system is discussed.

0 (Antibodies, Anti-Idiotypic), 0 (Antibodies, Monoclonal), 0 (Epitopes), 0 (Immunoglobulin G), 0 (Immunoglobulin Idiotypes), Animals, Antibodies, Anti-Idiotypic/*immunology, Antibodies, Monoclonal/biosynthesis/*immunology, Binding, Competitive/immunology, Cats, Cross Reactions/immunology, Dog Diseases/*immunology, Dogs, Enzyme-Linked Immunosorbent Assay, Epitopes/*immunology, Immunoglobulin G/analysis, Immunoglobulin Idiotypes/immunology, Mice, Mice, Inbred BALB C, Parvoviridae Infections/*veterinary, Parvoviridae/*immunology, Species Specificity,
Veterinary Immunology and Immunopathology
Erasmus MC: University Medical Center Rotterdam

Rimmelzwaan, G.F, van Es, J, Drost, G.A, Uytdehaag, F.G.C.M, & Osterhaus, A.D.M.E. (1991). Induction and characterization of monoclonal anti-idiotypic antibodies reactive with idiotopes of canine parvovirus neutralizing monoclonal antibodies. Veterinary Immunology and Immunopathology, 29(1-2), 139–150. doi:10.1016/0165-2427(91)90059-L