The studies of neuropsychiatric disorders would be facilitated by enhanced knowledge of the dorsolateral prefrontal cortex (DLPFC) proteome. To construct a data set of human DLPFC proteins, protein extracts were prepared from 12 postmortem brains focussing on the DLPFC region (Brodmann area 9) and analyzed using a combined gel electrophoresis and shotgun mass spectrometry approach, featuring data-independent label-free nanoflow liquid chromatography mass spectrometry (nLC-MSE). The detected mass/time features were aligned and annotated using the results from ProteinLynx Global Server. The resulting data set comprised 488 unique and accurately identified proteins, with stringent identification by a minimum of two distinct peptides detected at least in >75% of samples. These proteins were involved predominantly in cytoskeletal architecture, metabolism, transcription/translation, and synaptic function. Combination of this data set with that obtained by our previous characterization of the same brain region results in a total of 755 unique proteins, making this the most comprehensive analysis of this important brain region to date.

Biomedicine, Brain, Gel electrophoresis, MS, Synapse,
Erasmus MC: University Medical Center Rotterdam

Martins-de-Souza, D, Guest, P.C, Steeb, H, Pietsch, S, Rahmoune, H, Harris, L.W, & Bahn, S. (2011). Characterizing the proteome of the human dorsolateral prefrontal cortex by shotgun mass spectrometry. Proteomics, 11(11), 2347–2353. doi:10.1002/pmic.201000718