Human HLA class I- and HLA class II-restricted cloned cytotoxic T lymphocytes identify a cluster of epitopes on the measles virus fusion protein.
Journal of Virology , Volume 67 p. 2276- 2284
The transmembrane fusion (F) glycoprotein of measles virus is an important target antigen of human HLA class I- and class II-restricted cytotoxic T lymphocytes (CTL). Genetically engineered F proteins and nested sets of synthetic peptides spanning the F protein were used to determine sequences of F recognized by a number of F-specific CTL clones. Combined N- and C-terminal deletions of the respective peptides revealed that human HLA class I and HLA class II-restricted CTL efficiently recognize nonapeptides or decapeptides representing epitopes of F. Three distinct sequences recognized by three different HLA class II (DQw1, DR2, and DR4/w53)-restricted CTL clones appear to cluster between amino acids 379 and 466 of F, thus defining an important T-cell epitope area of F. Within this same region, a nonamer peptide of F was found to be recognized by an HLA-B27-restricted CTL clone, as expected on the basis of the structural homology between this peptide and other known HLA-B27 binding peptides.
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|Journal of Virology|
|Organisation||Erasmus MC: University Medical Center Rotterdam|
van Binnendijk, R.S, Versteeg-van Oosten, J.P.M, Poelen, M.C.M, Brugghe, H.F, Hoogerhout, P, Osterhaus, A.D.M.E, & Uytdehaag, F.G.C.M. (1993). Human HLA class I- and HLA class II-restricted cloned cytotoxic T lymphocytes identify a cluster of epitopes on the measles virus fusion protein. Journal of Virology, 67, 2276–2284. Retrieved from http://hdl.handle.net/1765/3461