In order to monitor the antibody response to feline immunodeficiency virus (FIV) in cats, following experimental and natural infection, enzyme-linked immunosorbent assays (ELISAs) were developed using recombinant env and gag proteins and p24-specific monoclonal antibodies. It was shown that in experimentally infected cats an env protein-specific antibody response was directly followed by a gag protein-specific response. Furthermore, an ELISA for the detection of env protein-specific serum antibodies proved more sensitive in identifying experimentally and naturally infected cats than ELISAs demonstrating gag protein-specific antibodies. It was concluded that, like in HIV infection of humans, the detection of env protein-specific serum antibodies in addition to gag protein-specific antibodies is not only an important tool in the diagnosis of the infection but also in studies concerning the pathogenesis of the disease.

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Veterinary Microbiology
Erasmus MC: University Medical Center Rotterdam

Rimmelzwaan, G., Siebelink, K., Broos, H., Drost, G. A., Weijer, K., van Herwijnen, R., & Osterhaus, A. (1994). Gag- and env-specific serum antibodies in cats after natural and experimental infection with feline immunodeficiency virus. Veterinary Microbiology, 39(1-2), 153–165. doi:10.1016/0378-1135(94)90096-5