Randomly amplified polymorphic DNA (RAPD), pulsed-field gelelectrophoresis (PFGE), and amplified fragment length polymorphism (AFLP) analyses were used to investigate a possible outbreak of Nocardia farcinica. RAPD and PFGE analyses yielded irreproducible and unsatisfactory results, respectively. AFLP analysis seem to be a promising and welcome addition for molecular analysis of Nocardia isolates. Copyright

dx.doi.org/10.1128/JCM.00932-07, hdl.handle.net/1765/35060
Journal of Clinical Microbiology
Erasmus MC: University Medical Center Rotterdam

Kalpoe, J.S, Templeton, K.E, Horrevorts, A.M, Endtz, H.P, Kuijper, E, Bernards, A.T, & Klaassen, C.H. (2007). Molecular typing of a suspected cluster of Nocardia farcinica infections by use of randomly amplified polymorphic DNA, pulsed-field gel electrophoresis, and amplified fragment length polymorphism analyses. Journal of Clinical Microbiology, 45(12), 4048–4050. doi:10.1128/JCM.00932-07