2007-10-01
Multicenter comparison of molecular methods for detection of Legionella spp. in sputum samples
Publication
Publication
Journal of Clinical Microbiology , Volume 45 - Issue 10 p. 3390- 3392
Legionellosis can be diagnosed by PCR using sputum samples. In this report, the methods of nine laboratories for 12 sputum samples with Legionella pneumophila and Legionella longbeachae are compared. We conclude that (i) liquefaction prevents PCR inhibition, (ii) the employed mip gene PCRs detected L. pneumophila only, and (iii) the 16S rRNA gene PCR detected both Legionella species and is preferred for the diagnosis of legionellosis. Copyright
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doi.org/10.1128/JCM.00505-07, hdl.handle.net/1765/35158 | |
Journal of Clinical Microbiology | |
Organisation | Erasmus MC: University Medical Center Rotterdam |
Bencini, M. A., van den Brule, A. J. C., Claas, E., Hermans, M., Melchers, W., Noordhoek, G. T., … Jansen, R. (2007). Multicenter comparison of molecular methods for detection of Legionella spp. in sputum samples. Journal of Clinical Microbiology, 45(10), 3390–3392. doi:10.1128/JCM.00505-07 |